Journal article
Aaron E. Chiou, Joshua A. Hinckley, Rupal Khaitan, Neta Varsano, Jonathan Wang, Henry F Malarkey, C. Hernandez, Rebecca M. Williams, L. Estroff, S. Weiner, L. Addadi, U. Wiesner, C. Fischbach
Small, 2020
Small, 2020
Semantic Scholar
DOI
PubMed
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APA
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Chiou, A. E., Hinckley, J. A., Khaitan, R., Varsano, N., Wang, J., Malarkey, H. F., … Fischbach, C. (2020). Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments. Small.
Chicago/Turabian
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Chiou, Aaron E., Joshua A. Hinckley, Rupal Khaitan, Neta Varsano, Jonathan Wang, Henry F Malarkey, C. Hernandez, et al. “Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments.” Small (2020).
MLA
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Chiou, Aaron E., et al. “Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments.” Small, 2020.
BibTeX Click to copy
@article{aaron2020a,
title = {Fluorescent Silica Nanoparticles to Label Metastatic Tumor Cells in Mineralized Bone Microenvironments.},
year = {2020},
journal = {Small},
author = {Chiou, Aaron E. and Hinckley, Joshua A. and Khaitan, Rupal and Varsano, Neta and Wang, Jonathan and Malarkey, Henry F and Hernandez, C. and Williams, Rebecca M. and Estroff, L. and Weiner, S. and Addadi, L. and Wiesner, U. and Fischbach, C.}
}
Abstract
During breast cancer bone metastasis, tumor cells interact with bone microenvironment components including inorganic minerals. Bone mineralization is a dynamic process and varies spatiotemporally as a function of cancer-promoting conditions such as age and diet. The functional relationship between skeletal dissemination of tumor cells and bone mineralization, however, is unclear. Standard histological analysis of bone metastasis frequently relies on prior demineralization of bone, while methods that maintain mineral are often harsh and damage fluorophores commonly used to label tumor cells. Here, fluorescent silica nanoparticles (SNPs) are introduced as a robust and versatile labeling strategy to analyze tumor cells within mineralized bone. SNP uptake and labeling efficiency of MDA-MB-231 breast cancer cells is characterized with cryo-scanning electron microscopy and different tissue processing methods. Using a 3D in vitro model of marrow-containing, mineralized bone as well as an in vivo model of bone metastasis, SNPs are demonstrated to allow visualization of labeled tumor cells in mineralized bone using various imaging modalities including widefield, confocal, and light sheet microscopy. This work suggests that SNPs are valuable tools to analyze tumor cells within mineralized bone using a broad range of bone processing and imaging techniques with the potential to increase the understanding of bone metastasis.
